Ectomycorrhizal symbionts and morpho-anatomical structure of Pinus sylvestris L. fine roots in clear-cuts of blueberry pine forests, middle taiga, the Komi Republic
T.A. Sizonenko, D.M. Shadrin
Section: Population ecology
We studied the features of mycorrhiza formation in Scots pine (Pinus sylvestris L.) in the 2008 and 2015 years clear-cuts of the blueberry pine forest of the middle taiga. The data on the morphology, anatomy of ectomycorrhizae, and molecular identification of fungal symbionts were obtained. Using light microscopy we detected 10 subtypes of fungal mantles, the occurrence and richness of which varied across community types and sampling years. Differences in quantitative parameters of the ectomycorrhizal roots were reliable in most cases. At the control site, the parameters characterizing the plant symbiotic component (ectomycorrhizae diameter, root diameter, and stele diameter) were significantly higher than at the nine years clear-cuts. At the same time, parameters of the fungal component (thickness and volume fraction of the fungal mantel, ectomycorrhizae density) were higher at the clear-cuts. For 42 of the 70 samples, we successfully performed fungal DNA isolation, amplification and ITS-region sequencing. Totally we identified 15 mycorrhizal fungi species from five families belonging to the Basidiomycota, and one species of Cenococcum geophilum belonged to the Ascomycota. Two species, Tylospora fibrillosa and Cenococcum geophilum, occurred at all study sites. A high occurrence was also found for Suillus variegatus, which forms tuberculated well-recognised pine ectomycorrhizae. The greatest diversity of mycorrhizal fungi isolated from pine roots and fungal mantel subtypes was found at the 2008 clear-cut. The species most frequently encountered in all study sites were those of the Russulaceae family. The blueberry pine forest and the 2008 clear-cut were the most similar in species composition.
ISSN 1995-4301
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