Stripping voltammetric deterction of antioxidants by the degree of reduction in the peak area of hydrogen peroxide
A.I. Fokina, M.A. Zaitsev, S.S. Kozachok, M.Yu. Kukhno
Section: Methodology and research methods. Models and forecasts
Currently the oxidative stress theory is a key to explaining the mechanisms of negative environmental impacts on human health. This led to growing interest in studying the antioxidant properties of various food products, dietary supplements, and pharmaceuticals. Plant-based materials and products are of particular focus. Plants that have long established themselves as sources of valuable biologically active compounds, including those with antioxidant properties, grow on the territory of the Kirov Oblast. Such plants include various species of conifers and Hippophae rhamnoides L. Electrochemical methods, particularly stripping voltammetry deserve priority attention as they allow simulation of conditions close to those in a living organism. The aim of this work was to study the possibility of detecting antioxidants (AOs) in a phosphate buffer solution (pH = 6.8) in the presence of hydrogen peroxide using a rotating disk electrode. The metrological characteristics of the method for AOs detecting in ethanol solutions were established by the standard addition method based on the degree of reduction of the hydrogen peroxide peak on the voltammogram after AOs addition. The obtained data and proposed methodological approaches can be useful in environmental studies of the antioxidant activity of various plant objects and in the interpretation of results, since they allow to assess both the value of the raw material as a source of AOs and various factors affecting the plants state.
ISSN 1995-4301
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